PCE 贝莱斯芽孢杆菌ES-120的生防与促生双重功能研究

Dual Roles of Bacillus velezensis ES-120 in Plant Disease Suppression and Growth Promotion

（1）Beneficial plant-associated microbes such as Bacillus species have important potential uses as bioinoculants that reduce dependence on agricultural chemicals.像芽孢杆菌这类对植物有益的相关微生物，作为能减少对农用化学品依赖的生物菌剂，具有重要的应用潜力。

（2）These microbes produce diverse metabolites that enhance plant resilience, but the functions of these compounds and the associated plant hormone pathways remain unclear.这些微生物会产生多种增强植物抗逆性的代谢物，但这些化合物的功能以及相关的植物激素通路尚不清楚。

（3）Using Bacillus velezensis ES-120, a novel strain isolated from Chenopodium album L., we demonstrate that distinct classes of ES-120 metabolites play different roles.我们利用从藜（Chenopodium album L.）中分离到的新型菌株贝莱斯芽孢杆菌 ES-120，证明了 ES-120 产生的不同类别代谢物发挥着不同的作用。

（4）Using Arabidopsis thaliana, we show that non-volatile soluble metabolites produced by ES-120, including lipopeptides identified by reversed-phase high-performance liquid chromatography, exhibit biocontrol activity by inhibiting multiple soil-borne pathogens.通过在拟南芥上的实验，我们发现 ES-120 产生的非挥发性可溶性代谢物，包括通过反相高效液相色谱鉴定出的脂肽类物质，通过抑制多种土传病原菌而表现出生物防治活性。

（5）In contrast, volatile organic compounds (VOCs) emitted by ES-120 promote plant growth, likely by modulating auxin and brassinosteroid (BR) signalling pathways, with effects sustained across diverse conditions.相比之下，ES-120释放的挥发性有机化合物则能促进植物生长，其作用可能是通过调控生长素和油菜素内酯信号通路实现的，并且这种效应在不同条件下都能持续。

（6）Headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry identified multiple VOCs produced by ES-120.采用顶空固相微萃取结合气相色谱-质谱联用技术，鉴定出了 ES-120 产生的多种挥发性有机化合物。

（7）Individual compounds showed limited activity, suggesting that growth promotion may depend on interactions among multiple VOCs or on an undetected low-abundance compound.单个化合物的活性有限，这表明促生长作用可能依赖于多种挥发性有机物之间的相互作用，或依赖于某种未被检出的低丰度化合物。

（8）In greenhouse trials, ES-120 reduced wilt in tomato (Solanum lycopersicum) and increased the biomass of Chinese cabbage (Brassica rapa var. chinensis).在温室试验中，ES-120减轻了番茄（Solanum lycopersicum）的青枯病，并增加了小白菜（Brassica rapa var. chinensis）的生物量。

（9）Thus, B. velezensis ES-120 functions as a dual-purpose bioinoculant: its soluble metabolites suppress soil-borne pathogens and its VOCs enhance plant growth via auxin and BR pathways, highlighting its potential for sustainable agriculture因此，贝莱斯芽孢杆菌 ES-120 是一种双功能的生物菌剂：其可溶性代谢物能抑制土传病原菌，而其挥发性有机物则通过生长素和油菜素内酯通路促进植物生长。这凸显了其在可持续农业中的应用潜力。

（10） Result

（11） B. velezensis ES-120 Exhibits Significant Biocontrol Activity Against Soil-Borne Pathogens B.velezensis ES-120对土传病原菌表现出显著的生物防治活性

（12） Cell-Free Supernatant From B. velezensis ES-120 Inhibits the Mycelial Growth of Pathogenic Fungi贝莱斯芽孢杆菌ES-120的无细胞上清液抑制病原真菌的菌丝生长

（13） Lipopeptides Are Among the Antimicrobial Compounds Secreted by ES-120 脂肽类物质是ES-120分泌的抗菌化合物之一

（14） VOCs Produced by B. velezensis ES-120 Promote Plants Growth贝莱斯芽孢杆菌ES-120产生的挥发性有机物促进植物生长

（15） Promotion of Arabidopsis Growth by ES-120 VOCs Involves the Auxin and BR Pathways ES-120挥发性有机物促进拟南芥生长涉及生长素和油菜素内酯通路

（16） Growth Promotion by ES-120 VOCs Is Not Driven by a Single Compound ES-120挥发性有机物的促生长作用并非由单一化合物驱动

（17） Growth-Promoting ES-120 VOCs Are Effective at Low Concentrations and Across Diverse Culture Media and Temperatures, but Require Light ES-120促生长挥发性有机物在低浓度、多种培养基和温度条件下均有效，但需要光照

（18） B. velezensis ES-120 Is a Promising Candidate for Agricultural Disease Management and Plant Growth Promotion贝莱斯芽孢杆菌ES-120是农业病害防控与植物促生的一个有前景的候选菌株

（1）Beneficial plant-associated microbes such as Bacillus species have important potential uses as bioinoculants that reduce dependence on agricultural chemicals.像芽孢杆菌这类对植物有益的相关微生物，作为能减少对农用化学品依赖的生物菌剂，具有重要的应用潜力。

（2）These microbes produce diverse metabolites that enhance plant resilience, but the functions of these compounds and the associated plant hormone pathways remain unclear.这些微生物会产生多种增强植物抗逆性的代谢物，但这些化合物的功能以及相关的植物激素通路尚不清楚。