（1） 核心问题：长期单一栽培和不规范的种植模式，导致人工栽培的黄连比野生黄连更容易生病。通过“再野化”（rewilding）——即恢复栽培植物根际的原始微生物群落——来促进植物健康生长是一个新策略，但适合黄连的原始微生物组成尚不清楚。

（2）研究发现（真菌组成）：具体来说，野生黄连根际的有益真菌比例更高，而栽培黄连根际的致病真菌比例更高。 

（3）关键有益菌属：研究进一步发现，一个公认的植物促生根际细菌属——副伯克霍尔德氏菌属（Paraburkholderia），在野生黄连抵抗由病原菌Ilyonectria引起的疾病中起着关键作用。 菌株分离与验证：研究人员分离到两株副伯克霍尔德氏菌（P. nemoris 和 P. phytofirmans）。实验室测试证明，这些菌株具有多种促进植物生长的特性，并能有效抑制引起黄连根腐病的已知病原菌。

摘要

（1）The sustained monoculture and irregular planting practices rendered the cultivated Coptis chinensis more prone to various diseases compared to its wild counterparts. Rewilding the rhizomicrobiome of cultivated plants has emerged as a promising strategy to promote plant growth, but ancestral microbiota suitable for C. chinensis remain largely uncharted. 与野生黄连相比，持续的单一栽培和不规则的种植做法使栽培黄连更容易患各种疾病。改造栽培植物的根茎微生物群落已成为促进植物生长的一种有前途的策略，但适合中国苦草的原始微生物群仍未被发现。

（2）The amplicon data analyses revealed that habitat transition strongly influenced the rhizosphere microbial communities. 扩增子数据分析显示，生境转换强烈影响根际微生物群落。 

（3）The rhizomicrobiomes of wild C. chinensis encompassed a more diverse array of ecological groups and exhibited a greater functional diversity compared to their cultivated counterparts. 与栽培物种相比，野生品种的根状茎生物群落包含了更多样的生态类群，表现出更大的功能多样性。

（4）A higher proportion of beneficial fungi was observed in the rhizosphere of wild C. chinensis, while the cultivated plants had a higher population of pathogenic fungi. 野生品种根际有益真菌比例较高，而栽培香椿根际致病真菌比例较高。

（5）Furthermore, a well-documented plant-growth-promoting rhizobacterium genus, Paraburkholderia, was found to play an essential role in the resistance of the wild C. chinensis to potential disease caused by Ilyonectria. 此外，一种被充分证明的促进植物生长的根际细菌属，Paraburkholderia，被发现在野生品种对由Ilyonectria引起的潜在疾病的抗性中起重要作用。

（6）Two strains of Paraburkholderia (Paraburkholderia nemoris and Paraburkholderia phytofirmans) were isolated, and in vitro experiments confirmed that these isolates possess various growth-promoting properties and antagonistic activities against known pathogens for C. chinensis root rot. 分离出两株类伯克霍尔德氏菌(nemoris类伯克霍尔德氏菌和phytofirmans类伯克霍尔德氏菌),体外实验证实这些分离物具有多种促生长特性和对已知的根腐病菌的拮抗活性。

（7）Both of the Paraburkholderia isolates could markedly promote the plant immune response and enhance the overall health of the cultivated C. chinensis. 两株类伯克霍尔德氏菌均可显著促进植物的免疫反应。

（8）结果

（9）Distinct rhizosphere microbial communities between wild and cultivated C. chinensis 野生和栽培品种根际微生物群落的差异

（10） Rhizosphere microbial communities of wild C. chinensis exhibit a greater functional diversity 野生品种根际微生物群落表现出较大的功能多样性

（11） Identification of differential microbial taxa in wild and cultivated C. chinensis 野生和栽培品种中不同微生物类群的鉴定

（12） Paraburkholderia strains from wild C. chinensis have growth-promoting and pathogen-antagonistic activities 来源于野生品种的类伯克霍尔德氏菌菌株具有促生长和拮抗病原菌的活性

（13） Paraburkholderia strains promote the growth of C. chinensis 副伯克霍尔德氏菌促进C. chinensis 的生长

Schematic model for the identification and application of the plant-growth-promoting rhizobacterium, Paraburkholderia. The potential fungal pathogens were enriched in the rhizosphere of cultivated C. chinensis, while a higher abundance of a well-documented PGPR, Paraburkholderia, was observed in the wild plants. Paraburkholderia isolates exhibited plant-growth-promoting and pathogen-antagonism activities, and their inoculation to the rhizosphere of cultivated C. chinensis could significantly promote C. chinensis growth 促进植物生长的根际细菌副伯克霍尔德氏菌的鉴定和应用模式。潜在的真菌病原体在栽培的黄连根际富集，而在野生植物中观察到更高丰度的PGPR，副伯克霍尔德氏菌。副伯克霍尔德氏菌具有促进植物生长和拮抗病原菌的活性，将其接种到栽培的品种根际可以显著促进其生长

The sustained monoculture and irregular planting practices rendered the cultivated Coptis chinensis more prone to various diseases compared to its wild counterparts. Rewilding the rhizomicrobiome of cultivated plants has emerged as a promising strategy to promote plant growth, but ancestral microbiota suitable for C. chinensis remain largely uncharted. 与野生黄连相比，持续的单一栽培和不规则的种植做法使栽培黄连更容易患各种疾病。改造栽培植物的根茎微生物群落已成为促进植物生长的一种有前途的策略，但适合 C. chinensis的原始微生物群仍未被发现。