然而，由于对宿主选择、微生物互作以及根际化学如何共同塑造病原菌抑制的理解尚不完整，基于微生物组的植物保护策略仍然受到限制。

Host-guided microbiome-metabolite interactions enable cross-kingdom SynComs for disease suppression

（1）The plant microbiome plays a crucial role in enhancing disease resistance, yet microbiome-based plant protection strategies remain limited by an incomplete understanding of how host selection, microbial interactions, and rhizosphere chemistry jointly shape pathogen suppression. 植物微生物组在增强抗病性方面发挥着至关重要的作用，然而，由于对宿主选择、微生物互作以及根际化学如何共同塑造病原菌抑制的理解尚不完整，基于微生物组的植物保护策略仍然受到限制。

（2）Here, we adopt a “learning from nature” approach to design synthetic microbial communities (SynComs) that recapitulate naturally evolved disease-suppressive interactions, using banana Fusarium wilt as a model system. 在此，我们采用了一种“向自然学习”的策略，以香蕉枯萎病为模型体系，设计了能够再现自然进化中病害抑制互作的合成微生物群落。

（3）High-throughput profiling revealed that both bacterial and fungal communities contribute to varietal resistance.高通量分析揭示，细菌和真菌群落均对品种抗性有贡献。

（4）Resistance-associated microbial taxa were identified and isolated to assemble bacterial, fungal, and cross-kingdom SynComs representative of resistant versus susceptible hosts.通过鉴定和分离与抗性相关的微生物类群，我们组装了分别代表抗病和感病宿主的细菌、真菌以及跨界合成微生物群落。

（5）SynComs derived from resistant varieties suppressed pathogen growth more effectively than those from susceptible hosts, with cross-kingdom SynComs exhibiting the strongest effects.来源于抗病品种的合成微生物群落比来源于感病宿主的合成微生物群落更有效地抑制了病原菌的生长，其中跨界合成微生物群落的效应最强。

（6）Cross-kingdom SynCom inoculation significantly reduced disease severity and restructured both the composition and functional potential of the rhizosphere microbiome.接种跨界合成微生物群落显著降低了病害严重程度，并重塑了根际微生物群的组成和功能潜力。

（7）Integrative transcriptomic and metabolomic analyses revealed coordinated host metabolic reprogramming, characterized by increased accumulation of diverse metabolites, including alkaloids, amino acids, and flavonoids.整合的转录组学和代谢组学分析揭示了宿主发生协同的代谢重编程，其特征是多种代谢物（包括生物碱、氨基酸和黄酮类化合物）的积累增加。

（8）Notably, supplementation with resistance-associated rhizosphere metabolites, such as stearic acid and shikimic acid, further enhanced disease suppression.

值得注意的是，补充与抗性相关的根际代谢物（如硬脂酸和莽草酸）可进一步增强病害抑制效果。

（9）Together, our findings establish a mechanistic framework in which host-guided microbiome assembly and metabolite-mediated interactions jointly enable effective cross-kingdom SynComs for disease suppression, providing ecological principles for microbiome-based plant protection strategies. 综上所述，我们的发现建立了一个机制框架：宿主导向的微生物组组装与代谢物介导的相互作用共同促成了有效的跨界合成微生物群落的病害抑制能力，为基于微生物组的植物保护策略提供了生态学原理。

（10） Result

（11）Profiles of bacterial and fungal communities across banana varieties不同香蕉品种间细菌和真菌群落的分布特征

（12）Rhizosphere microbial communities and metabolites compositions differing across banana varieties 不同香蕉品种间根际微生物群落及代谢物组成的差异

（13）Cultivation of bacterial and fungal isolates from rhizosphere soils根际土壤中细菌和真菌分离株的培养

（14）Pathogen suppression potential of selected bacterial and fungal strains 所选细菌和真菌菌株的病原菌抑制潜力

（15）Cross-kingdom SynComs from the highly resistant variety effectively suppress pathogen 来自高抗品种的跨界合成微生物群落有效抑制病原菌

（16）SynCom treatment alters rhizosphere microbial community composition and function 合成微生物群落处理改变根际微生物群落的组成与功能

（17）SynCom treatment induces distinct transcriptomic and metabolic changes in banana roots合成微生物群落处理诱导香蕉根系产生独特的转录组和代谢组变化

（18）Addition of metabolites from the highly resistant variety enhanced SynCom-mediated pathogen suppression添加高抗品种的代谢物增强了合成微生物群落介导的病原菌抑制效果

添加高抗品种的代谢物增强了合成微生物群落介导的病原菌抑制效果