方法：在此，我们对13项研究进行了荟萃分析，这些研究包括了来自52个植物宿主的3000多个根部和非根际土壤样本，旨在识别受干旱持续影响的细菌类群，并将其与接种结果联系起来。

A conserved bacterial signature characterizes plant microbiome responses to drought

（1）Introduction: Plant-associated microbes contribute to host resilience under stress, yet the extent to which microbial responses to drought generalize across hosts and environments remains unclear. 引言：与植物相关的微生物有助于宿主在胁迫下的恢复力，但微生物对干旱的响应在多大程度上能够在不同宿主和环境之间普遍适用，仍不清楚。

（2） Methods: Here, we performed a meta-analysis of 13 studies including more than 3,000 root and bulk soil samples from 52 plant hosts to identify bacterial taxa consistently affected by drought and link them to inoculation outcomes. 方法：在此，我们对13项研究进行了荟萃分析，这些研究包括了来自52个植物宿主的3000多个根部和非根际土壤样本，旨在识别受干旱持续影响的细菌类群，并将其与接种结果联系起来。

（3） Using a standardized processing workflow and differential abundance analysis, we derived a “drought signature” of taxa differentially abundant under water limitation across the endosphere, rhizosphere, and bulk soil. 通过标准化的处理流程和差异丰度分析，我们得出了一个“干旱特征”，即在内生圈、根际圈和非根际土壤中水分限制条件下差异丰度的类群。

（4） The signature is dominated by Gram-positive Actinobacteria enriched under drought, including Kribella, and by Gram-negative taxa depleted under drought, such as Ramilbacter. 该干旱特征以在干旱条件下富集的革兰氏阳性放线菌（包括Kribella属）和在干旱条件下减少的革兰氏阴性菌类群（如Ramilbacter属）为主。

（5） Comparison with four independent inoculation experiments revealed limited overlap between drought and inoculation responses, with only Nitrospira depleted in both contexts. 与四个独立的接种实验比较显示，干旱响应与接种响应之间的重叠有限，仅有硝化螺旋菌属在两种情况下均减少。

（6） Discussion: Studies reporting improved plant performance under inoculation also exhibited stronger and more extensive microbial shifts, suggesting that the magnitude of community restructuring may be characteristic of successful inoculation outcomes. 报告接种下植物性能改善的研究也表现出更强、更广泛的微生物变化，这表明群落重构的幅度可能是成功接种结果的特征。

（7） This work provides the first genus-level meta-analysis of microbial responses under drought and inoculation, across hosts and experimental conditions, and delivers a unified root and soil microbiome dataset. 这项工作首次提供了在不同宿主和实验条件下，干旱和接种下微生物响应的属水平荟萃分析，并提供了一个统一的根部和土壤微生物组数据集。

（8）Result

（9）A data integration pipeline for root and soil drought microbiome studies 根和土壤干旱微生物组研究的数据集成管道

（10） Large-scale dataset reveals patterns in microbial composition across studies and plant hosts 大规模数据集揭示了跨研究和植物宿主的微生物组成模式

展示了不同植物类群和不同生态位中微生物群落的组成与核心微生物

（11） Across plant hosts and experimental conditions, microbial communities reveal a shared drought footprint 跨越植物宿主和实验条件，微生物群落揭示了一个共同的干旱足迹

展示了干旱胁迫下不同生态位中微生物属的差异丰度变化（干旱特征），并验证了这一特征在独立研究中的一致性。

（12） Relation between drought signatures and inoculation response 干旱信号与接种反应的关系

这张图展示了在四个独立的接种实验中，干旱特征微生物属的响应情况，用来回答：干旱下富集/减少的微生物，在接种后是否也会发生变化？

与植物相关的微生物有助于宿主在胁迫下的恢复力，但微生物对干旱的响应在多大程度上能够在不同宿主和环境之间普遍适用，仍不清楚。