（1）与根际定殖相关的基因（如flagellin、chemotaxis、biofilm formation等）在LHJ6中显著高表达，表明该菌具有在根际稳定定殖的遗传基础

摘要

（1） Panax notoginseng (P. notoginseng), a medicinal herb rich in bioactive saponins derived from its roots, exhibits diverse pharmacological activities relevant to cardiovascular diseases. 三七是一种药用植物，其根部富含具有生物活性的皂苷，表现出与心血管疾病相关的多种药理活性。

（2）Root rot, a devastating soil-borne disease, threatens the sustainability of P. notoginseng production.  根腐病是一种毁灭性的土传病害，威胁着三七生产的可持续性。

（3） Biological control is a green and effective approach for disease control.  生物防治是一种绿色有效的病害防控方法。

（4） Herein, we successfully isolated a biocontrol strain, Paenibacillus maysiensis (P. maysiensis) LHJ6, from the rhizosphere of healthy P. notoginseng. 在此，我们从健康三七的根际成功分离出一株生防菌株——玉米类芽孢杆菌 LHJ6。

（5）Pot experiments demonstrated that LHJ6 reduced root rot incidence by 80.0% and the disease index by 75.0%, with significant efficacy observed under field conditions. 盆栽实验表明，LHJ6使根腐病发病率降低了80.0%，病情指数降低了75.0%，并且在田间条件下也观察到了显著的防治效果。

（6） Whole-genome sequencing revealed multifaceted mechanisms underlying biological control and plant growth promotion. 全基因组测序揭示了该菌株生物防治和促进植物生长的多方面机制。

（7） Genomic analysis revealed a single circular chromosome spanning 6.13 Mb, with 5,886 protein-coding genes.  基因组分析显示，该菌株有一个长度为6.13 Mb的单一环状染色体，包含5,886个蛋白质编码基因。

（8） LHJ6 exerted its potent biocontrol activity against plant pathogens through multiple mechanisms, including rhizosphere colonisation, production of diverse antifungal metabolites, degradation of pathogenic fungal cell walls, siderophore production, nitrogen fixation, and potential induction of systemic resistance in host.  LHJ6通过多种机制发挥其对植物病原菌的强效生防活性，包括根际定殖、产生多种抗真菌代谢物、降解病原真菌细胞壁、产生铁载体、固氮以及可能诱导宿主的系统抗性。

（9） These findings offer fundamental insights into the biocontrol mechanisms of P. maysiensis strain LHJ6, providing a foundation for developing sustainable agricultural applications in P. notoginseng cultivation. 这些发现为理解玉米类芽孢杆菌 LHJ6 的生防机制提供了基础性见解，为开发三七种植中的可持续农业应用奠定了基础。

（10）Result

（11） Sample Collection and Antagonistic Strain Screening 样品采集和拮抗菌株筛选

（12） Morphological and Molecular Identification of LHJ6 LHJ6的形态和分子鉴定

（13） Assessment of the Antimicrobial Activity 抗菌活性的评估

（14） Genomic Analysis 基因组分析

（15） Analysis of CAZymes and Biosynthetic Potential of Antimicrobial Metabolites in Strain LHJ6 菌株LHJ6抗菌代谢产物的酶学和生物合成潜力分析

（16） Characterisation of Hydrolase Activity, Plant Growth-Promoting Traits and Colonisation-Related Gene Expression in Strain LHJ6 菌株LHJ6中水解酶活性、植物生长促进性状和定殖相关基因表达的特征

（17） Comparative Genomic Analysis 比较基因组分析

（18） Genes Associated With PGP Traits and Potent Antagonistic Activity in the LHJ6 Genome LHJ6基因组中与PGP性状和有效拮抗活性相关的基因

对菌株LHJ6基因组中生防与促生相关基因的系统梳理，从根际定殖、生物膜形成、营养竞争、抗菌物质合成、植物生长调节等多个方面，解释了该菌株为什么能有效防治三七根腐病并促进植物生长。

 盆栽实验表明，LHJ6使根腐病发病率降低了80.0%，病情指数降低了75.0%，并且在田间条件下也观察到了显著的防治效果。