沙漠环境具有极端的生存条件，包括高温、干旱、强紫外线辐射、寡营养等。在这种恶劣环境下能够存活的植物（如本研究的枣树 Ziziphus lotus），本身具有极强的抗逆性。而它们的根内圈（endosphere）共生着大量微生物，这些微生物与宿主植物长期共进化，很可能也获得了帮助植物抵抗多重胁迫的能力。

摘要

（1）Ziziphus lotus (L.) Desf. is a resilient shrub native to Morocco's arid and semi-arid regions.Ziziphus lotus (L.) Desf. 是一种原产于摩洛哥干旱和半干旱地区的强健灌木。

（2）This study aimed to isolate and characterize plant growth-promoting bacteria (PGPB) from the root endosphere of Z. lotus, and to evaluate the most effective strain in planta, assessing its impact on plant germination, growth, and modification of the soil microbiota associated to wheat under greenhouse conditions.本研究旨在从Z. lotus 的根内圈中分离和鉴定具有植物生长促进活性的细菌，并在温室条件下评估最有效菌株对小麦萌发、生长及土壤微生物群落的影响。

（3）Our results showed that among the isolated endophytes, Bacillus subtilis ED24 demonstrated strong plant growth-promoting properties, including solubilization of insoluble phosphorus and zinc, nitrogen fixation, and siderophores production.我们的结果表明，在分离得到的内生菌中，枯草芽孢杆菌（Bacillus subtilis）ED24 表现出较强的植物促生特性，包括溶解不溶性磷和锌、固氮以及产铁载体。

（4）Findings from the pot experiment demonstrated that B. subtilis ED24 significantly improved germination (96% vs. 83% in control), shoot height (52.76 cm vs. 44.98 cm), leaf area (46.2 cm² vs. 30.82 cm²), and shoot biomass (4.28 g vs. 3.45 g).盆栽实验结果表明，B. subtilis ED24 显著提高了小麦的发芽率（96% vs. 对照83%）、株高（52.76 cm vs. 44.98 cm）、叶面积（46.2 cm² vs. 30.82 cm²）和地上部生物量（4.28 g vs. 3.45 g）。

（5）Inoculation also enhanced shoot potassium content (106.6 mg/pot vs. 63.92 mg/pot), grain yield and quality along with a slight increase in grain protein content (19.50% vs. 18.39%) and grain crude fiber content (2.53% vs. 2.48%).接种还提高了地上部钾含量（106.6 mg/盆 vs. 63.92 mg/盆）、籽粒产量和品质，同时籽粒蛋白质含量（19.50% vs. 18.39%）和粗纤维含量（2.53% vs. 2.48%）也略有增加。

（6）Furthermore, inoculation with B. subtilis ED24 enriched beneficial rhizospheric bacteria (e.g., Cicerobacter, Streptomyces, Ferrovibrio, Rhizobium) and reduced pathogenic fungi, such as Fusarium.此外，接种 B. subtilis ED24 还富集了有益根际细菌（如 Cicerobacter、链霉菌属、Ferrovibrio、根瘤菌属），并减少了病原真菌（如镰刀菌属）。

（7）These findings highlight the potential of B. subtilis ED24 to promote wheat growth and beneficially shape the wheat-associated microbial community. 这些发现凸显了 B. subtilis ED24 在促进小麦生长和有益塑造小麦相关微生物群落方面的潜力。

（8）Result

（9）Isolation of endophytic bacteria   内生细菌的分离

（10） 16S rRNA gene identification16S rRNA基因鉴定

（11） Plant growth promoting traits and B. subtilis ED24 selection    植物促生特性与枯草芽孢杆菌ED24的筛选

（12） Effect of B. subtilis ED24 on wheat germination and seedling parameters      枯草芽孢杆菌ED24对小麦萌发及幼苗参数的影响

（13） Effects of B. subtilis ED24 on wheat growth枯草芽孢杆菌ED24对小麦生长的影响

（14） Effect on wheat plant morphological parameters      对小麦植株形态参数的影响

（15） Effect on shoot N, P, K compositions   对地上部氮、磷、钾含量的影响

（16） Effect of B. subtilis ED24 on wheat yield and seeds compositions  枯草芽孢杆菌ED24对小麦产量及籽粒成分的影响

（17） Effect of B. subtilis ED24 on soil microbial community      枯草芽孢杆菌ED24对土壤微生物群落的影响

This study aimed to isolate and characterize plant growth-promoting bacteria (PGPB) from the root endosphere of Z. lotus, and to evaluate the most effective strain in planta, assessing its impact on plant germination, growth, and modification of the soil microbiota associated to wheat under greenhouse conditions.本研究旨在从Z. lotus 的根内圈中分离和鉴定具有植物生长促进活性的细菌，并在温室条件下评估最有效菌株对小麦萌发、生长及土壤微生物群落的影响。