无症状的抗性品种CL11 (CL11H)表现出富含防御化合物(灵芝酸I和L-异亮氨酸)的代谢特征，并包含由链霉菌属、纤维弧菌属主导的有益细菌群落。

而无症状的敏感品种ZN6 (ZN6H)显示出较高水平的初级代谢物(甲基丙二酸和琥珀酸)以及相对丰富的无枝酸菌属和黄杆菌属。、

在症状发展时，敏感症状ZN6 (ZN6D)产生“呼救”反应，积累L-色氨酸和柠檬酸，这与异根瘤菌-新根瘤菌-副根瘤菌-根瘤菌的补充相关。

相反，抗性症状CL11 (CL11D)部署了一个代谢模式(L-3-氰基丙氨酸、4-羟基苯甲酰葡萄糖、D-果糖)，其富集了芽孢杆菌和纤维弧菌。

Correlation between root exudate composition and beneficial bacteria underlies Fusarium wilt resistance in cucumber

（1）Rhizosphere microbes regulated by root exudates play important roles in promoting plant growth and suppressing soil-borne diseases 受根系分泌物调控的根际微生物在促进植物生长和抑制土传病害方面发挥着重要作用 . 

（2）However, their functions in monoculture systems are not well studied. 然而，它们在连作系统中的功能没有得到很好的研究。 

（3）Here, we integrated metabolomic and microbiome analyses to compare root exudate profiles and rhizosphere communities of Fusarium oxysporum f. sp. cucumerinum (Foc) resistant (CL11) and susceptible (ZN6) cultivars grown in naturally infested, continuous-cropping soil. The asymptomatic, resistant cultivar CL11 (CL11H) exhibited a metabolic signature enriched in defensive compounds (ganoderic acid I and L-isoleucine) and harbored a beneficial bacterial community dominated by Streptomyces, Cellvibrio, and Ensifer. 在这里，我们整合了代谢组学和微生物组分析来比较尖孢镰孢的根分泌物和根际群落。生长在自然感染的连作土壤中的黄瓜抗(CL11)和感(ZN6)品种。无症状的抗性品种CL11 (CL11H)表现出富含防御化合物(灵芝酸I和L-异亮氨酸)的代谢特征，并包含由链霉菌属、纤维弧菌属主导的有益细菌群落。

（4）While the asymptomatic susceptible cultivar ZN6 (ZN6H) showed elevated levels of primary metabolites (methylmalonic and succinic acids) and an enrichment in the relative abundance of Amycolatopsis and Flavobacterium. Upon symptom development, susceptible symptomatic ZN6 (ZN6D) mounted a "cry-for-help" response, accumulating L-tryptophan and citric acid, which correlated with recruitment of Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium. Conversely, resistant symptomatic CL11 (CL11D) deployed a metabolic profile (L-3-cyanoalanine, 4-hydroxybenzoyl glucose, D-fructose), which enriched Bacillus and Cellvibrio. 而无症状的敏感品种ZN6 (ZN6H)显示出较高水平的初级代谢物(甲基丙二酸和琥珀酸)以及相对丰富的无枝酸菌属和黄杆菌属。在症状发展时，敏感症状ZN6 (ZN6D)产生“呼救”反应，积累L-色氨酸和柠檬酸，这与异根瘤菌-新根瘤菌-副根瘤菌-根瘤菌的补充相关。相反，抗性症状CL11 (CL11D)部署了一个代谢模式(L-3-氰基丙氨酸、4-羟基苯甲酰葡萄糖、D-果糖)，其富集了芽孢杆菌和纤维弧菌。

（5）Exogenous application of citric acid, L-isoleucine, L-tryptophan, hexadecanedioic acid, and succinic acid to susceptible ZN6 plants significantly suppressed wilt disease 柠檬酸、L-异亮氨酸、L-色氨酸、十六烷二酸和琥珀酸对敏感的ZN6植物的外源施用显著抑制了枯萎病 . 

（6）These metabolites restructured the rhizosphere bacterial communities, demonstrated by enrichments of Pseudomonas, Burkholderia-Caballeronia-Paraburkholderia, and Comamonadaceae by L-tryptophan, as well as Pseudomonas and Bacillus promoted by L-isoleucine and citric acid, respectively. 这些代谢物重组了根际细菌群落，通过L-色氨酸富集假单胞菌属、伯克霍尔德菌属-卡瓦列罗菌属-副伯克霍尔德菌属和丛毛单胞菌科，以及分别由L-异亮氨酸和柠檬酸促进的假单胞菌属和芽孢杆菌属证明了这一点。  

（7）Moreover, succinic acid suppressed disease through microbiome-mediated mechanisms despite its in vitro positive effect on Foc growth. These findings demonstrate that root exudates are linked to cultivar-specific disease outcomes, and that target metabolite application can suppress Fusarium wilt through microbiome-mediated mechanisms or a combined antifungal mode. 此外，琥珀酸通过微生物介导的机制抑制疾病，尽管其在体外对Foc生长有积极作用。这些发现表明，根分泌物与品种特异性疾病结果相关，并且目标代谢物的应用可以通过微生物介导的机制或组合的抗真菌模式抑制枯萎病。

（8）Result

（9）Pathogen identification and sample collection for further metabolomic and rhizosphere bacterial community analyses 病原体鉴定和样品收集，用于进一步的代谢组学和根际细菌群落分析

（10） Differential root exudate profiles in resistant CL11 and susceptible ZN6 cucumber cultivars in response to Foc stress 黄瓜抗病品种CL11和感病品种ZN6根系分泌物差异对Foc胁迫的响应

（11） Differences of rhizosphere bacterial community of resistant CL11 and susceptible ZN6 抗病品系CL11和感病品系ZN6根际细菌群落的差异

（12） Correlation of differential bacterial taxa with specific root exudates of cultivars CL11 and ZN6 不同细菌类群与品种CL11和ZN6根系分泌物的相关性

（13） Specific root exudate compounds showed antifungal activity against Foc growth in vitro 特定的根分泌物化合物在体外表现出抗真菌活性

（14） Exogenous addition of root exudate compounds promotes disease suppression in susceptible cultivar ZN6 and resistant cultivar CL11 in vivo 根分泌物化合物的外源添加促进了感病品种ZN6和抗病品种CL11体内的病害抑制

（15） The application of root exudate compounds mediates disease suppression in susceptible cultivar ZN6 through the recruitment of rhizosphere bacteria 根分泌物化合物的应用通过根际细菌的补充介导了感病品种ZN6的病害抑制

（16）逻辑框架

Rhizosphere microbes regulated by root exudates play important roles in promoting plant growth and suppressing soil-borne diseases