（1）66% 的 ASV 在根、地上部和根际土壤中共享”——揭示的是一个非常强的生态信号：在极端高盐胁迫下，根际土壤中的嗜盐核心菌群，有能力穿过植物的防御系统，系统性地定殖到植物的根部乃至地上组织的内部。

（1）Coastal salt flats, locally known as sabkhas, are hypersaline, alkaline desert ecosystems that impose extreme abiotic stress on microbial and plant life. 沿海盐滩，当地称为“sabkhas”，是超盐碱性的沙漠生态系统，对微生物和植物生命施加着极端的环境胁迫。

（2）Despite their ecological significance, plant-associated microbiomes in these habitats remain poorly characterized.尽管这些栖息地具有重要的生态意义，但其中与植物相关的微生物组仍未得到充分的研究。

（3）In this study, we investigated the bacterial communities of native halophytes across three sabkha sites in southern Morocco using an integrated culture-independent and culture-dependent framework.在本研究中，我们采用非培养与培养相结合的综合方法，对摩洛哥南部三个sabkha位点的本土盐生植物相关细菌群落进行了调查。

（4）Soil physicochemical analyses revealed strong gradients in salinity and ionic composition, along with consistent alkaline pH across sites.土壤理化分析揭示了不同位点在盐度和离子组成上的强烈梯度变化，以及所有位点均呈现一致性的碱性pH值。

（5）These conditions strongly structured bacterial assemblage: alpha diversity declined progressively from bulk soil to rhizosphere soil, root, and shoot; and beta diversity showed clear compartmental separation driven by environmental factors.这些条件强烈地塑造了细菌群落的组成：从非根际土壤到根际土壤、根部和地上部，α多样性逐渐降低；而β多样性则显示出由环境因素驱动的明显的生态位分化。

（6）Canonical correspondence analysis identified electrical conductivity (EC), Na₂O, K₂O, and carbonate fractions as the main abiotic drivers.典型对应分析确定电导率、氧化钠、氧化钾和碳酸盐组分为主要非生物驱动因子。

（7）Across plant species, bacterial communities converged toward a stable halophilic core microbiome dominated by Halomonas, Kushneria, and Marinococcus, with 66% of amplicon sequencing variants (ASVs) shared across compartments.在不同植物物种中，细菌群落趋于形成一个稳定的嗜盐核心微生物组，以盐单胞菌属、Kushneria和海球菌属为主，其中66%的扩增子序列变异在不同生态位之间共享。

（8）Host identity played a secondary role as environmental filtering overshadowed host-specific associations.宿主身份起到次要作用，因为环境过滤的作用超过了宿主特异性关联。

（9）Culture-dependent isolation recovered 19 halophilic and halotolerant bacterial strains, including representatives of Halomonas, Idiomarina, Marinobacter, Psychrobacter, Planomicrobium, and Bacillus. 通过可培养方法分离获得了19株嗜盐和耐盐细菌菌株，包括盐单胞菌属、Idiomarina、海杆菌属、嗜冷杆菌属、Planomicrobium和芽孢杆菌属的代表菌株。

（10）These isolates exhibited robust growth on saline Marine Agar medium, indicating strong salt tolerance consistent with their occurrence in hypersaline environments.这些分离株在盐性海洋琼脂培养基上表现出强健的生长能力，表明其具有很强的耐盐性，与其在超盐环境中的分布一致。

（11）The strong concordance between cultured isolates and metabarcoding profile confirms that dominant halophilic lineages are both ecologically robust and readily culturable.分离培养菌株与扩增子测序结果之间的高度一致性证实，占主导地位的嗜盐微生物谱系既是生态上强健的，也是易于培养的。

（12）Together, these findings demonstrate that sabkha plant microbiomes are primarily shaped by deterministic abiotic filtering and harbor resilient, stress-adapted bacterial communities. 总之，这些发现表明，sabkha环境下的植物微生物组主要受确定性的非生物过滤作用塑造，并包含了具有韧性和适应胁迫能力的细菌群落。

（·13）Sabkhas thus represent promising reservoirs of halophilic microbes with potential applications in saline agriculture and improving crop resilience under extreme environmental conditions. 因此，sabkhas代表了有前景的嗜盐微生物资源库，在盐碱农业和提高作物在极端环境条件下的韧性方面具有潜在应用价值。

（14）Result

（15）Soil physicochemical properties in the rhizosphere soils reflected bulk soil salinity gradients in sabkha 根际土壤的理化性质反映了塞卜哈的土壤盐分梯度

（16）Alpha- and beta-diversity analyses reveal strong soil-plant filtering α和β多样性分析揭示了强大的土壤-植物过滤作用

（17）Bacterial diversity and richness respond to spatial distribution 细菌的多样性和丰富性与空间分布有关

（18）Indicator bacterial species reveal strong compartment- and host-specific signatures 指示细菌物种揭示了强烈的区室特异性和宿主特异性特征

（19）Core bacteriome and cross-host convergence 核心细菌组

（20）Environmental drivers of community structure 社区结构的环境驱动因素

（21）Culture-dependent isolation and identification of halophytic bacteria 盐生细菌的培养分离和鉴定

Despite their ecological significance, plant-associated microbiomes in these habitats remain poorly characterized.尽管这些栖息地具有重要的生态意义，但其中与植物相关的微生物组仍未得到充分的研究。