（1）水稻基因型 → 招募特定菌群 → 菌群分解有机质 → 产生氨基酸 → 氨基酸被根系吸收 → 提高氮肥利用效率和产量。

（2）之前已知，粳稻（japonica）比籼稻（indica）吸收氨基酸的能力更强，这归因于它们拥有的OsLHT1基因版本不同。粳稻的版本叫 OsLHT1a。

摘要

（1）Amino acids are plant-available organic nitrogen (N) that can be directly absorbed, but their availability relies on microbial decomposition of organic matter in the soil. 氨基酸是植物可直接吸收的有机氮形态，但其可利用性依赖于土壤中有机物的微生物分解。

（2）Natural variation in Lysine-Histidine-Type Transporter-1 (OsLHT1) (NCBI Gene ID: 3974662) is associated with higher amino acid uptake in japonica rice than in indica.赖氨酸-组氨酸型转运蛋白1（OsLHT1）的自然变异与粳稻比籼稻具有更高的氨基酸吸收能力相关。

（3）However, how this genetic variation influences rhizosphere microbiome assembly and its subsequent impact on amino acid acquisition remains unclear.然而，这种遗传变异如何影响根际微生物组的组装，以及其对氨基酸获取的后续影响，仍不清楚。

（4）In this study, we demonstrate that the OsLHT1a allele in japonica is prevalent in rice grown in high-organic-N soils, where it recruits a distinct rhizosphere microbiome to enhance amino acid acquisition.本研究表明，粳稻中的OsLHT1a等位基因在高有机氮土壤中种植的水稻中普遍存在，该基因通过招募独特的根际微生物组来增强氨基酸的获取。

（5）A synthetic microbiota composed of bacteria enriched by the OsLHT1a allele in japonica enhanced amino acid production in soil through organic matter decomposition and increased root amino acid uptake by upregulating OsLHT1 gene expression.由粳稻OsLHT1a等位基因富集的细菌组成的合成微生物群，通过有机物分解提高了土壤中氨基酸的产生，并通过上调OsLHT1基因表达增加了根系对氨基酸的吸收。

（6）The rhizosphere colonization of the synthetic microbiota was specifically driven by the function of OsLHT1.该合成微生物群在根际的定殖特异性地由OsLHT1的功能驱动。

（7）Notably, organic fertilization facilitated this colonization, thereby improving organic N use efficiency and rice yield.值得注意的是，有机施肥促进了这种定殖，从而提高了有机氮利用效率和水稻产量。

（8）This root–rhizosphere microbiome functional synergy under organic fertilization presents a promising strategy to increase organic fertilizer use efficiency and demonstrates the potential for harnessing plant-gene-associated rhizosphere microbiomes for sustainable agriculture.有机施肥下这种根系-根际微生物组的功能协同作用，为提高有机肥利用效率提供了一种有前景的策略，并展示了利用植物基因相关的根际微生物组促进可持续农业的潜力。

（9）Result

（10）Natural variation of OsLHT1 correlates with soil organic N availability and rhizosphere microbiome differentiation OsLHT1的自然变异与土壤有机氮有效性和根际微生物群落分化相关

（11）The OsLHT1a haplotype in japonica enriches amino-acid-acquisition-related rhizosphere bacteria in organic fertilized soil 粳稻OsLHT1a单倍型丰富了有机施肥土壤中与氨基酸获取相关的根际细菌

（12）OsLHT1 mediates the assembly of SynM OsLHT1介导SynM的组装

（13）OsLHT1 maintains the cross-generation influence of SynM OsLHT1保持了SynM的跨代影响力

（14）Field application of SynM promoted organic fertilizer utilization and increased rice yield 田间施用SynM促进了有机肥的利用，提高了水稻产量

OsLHT1a（粳稻型等位基因）通过招募合成菌群（SynM），这些菌群一方面分解有机质产生氨基酸，另一方面上调OsLHT1自身的表达，形成了一个“基因-微生物-营养”的正向循环，从而高效利用土壤有机氮。

（4）In this study, we demonstrate that the OsLHT1a allele in japonica is prevalent in rice grown in high-organic-N soils, where it recruits a distinct rhizosphere microbiome to enhance amino acid acquisition.本研究表明，粳稻中的OsLHT1a等位基因在高有机氮土壤中种植的水稻中普遍存在，该基因通过招募独特的根际微生物组来增强氨基酸的获取。

（5）A synthetic microbiota composed of bacteria enriched by the OsLHT1a allele in japonica enhanced amino acid production in soil through organic matter decomposition and increased root amino acid uptake by upregulating OsLHT1 gene expression.由粳稻OsLHT1a等位基因富集的细菌组成的合成微生物群，通过有机物分解提高了土壤中氨基酸的产生，并通过上调OsLHT1基因表达增加了根系对氨基酸的吸收。

（6）The rhizosphere colonization of the synthetic microbiota was specifically driven by the function of OsLHT1.该合成微生物群在根际的定殖特异性地由OsLHT1的功能驱动。